In this case, recovery and purified times of sod-iiare the highest in the three same functional enzymes 由此可知利用此法sod-纯化倍数及活性回收率在三个同工酶中最高。
Fourthly, according to the activity, collect, dialyse, freeze, dry respectively the sod protein through deae-sepharose column chromatog-raphy; process the sod protein through sephacryl s-200 column chromatography with the preceding method . at last, process the pure sod protein with same functional enzyme electrophoresis and active dye, isoelectric focusing electrophoresis and sds-page 将粗酶液过deae-琼脂糖柱层析,得三个活力峰,分别收集、透析、干燥浓缩后;再上sephacryls-200凝胶柱层析,按与deae-琼脂糖柱层析后同样的方法收集处理。